Fig. 4: CNMT-dependent promotion of ferroptosis by PQS in macrophages.

A CCK-8 assay for cell viability in RAW264.7, shCNMT, and shCNMT + (shCNMT expressing CNMT from a rescue construct pCMV-CNMT) macrophages treated with PQS for 24 h. Data are presented as mean ± SD (n = 3, two-way ANOVA with Tukey’s multiple comparisons test). B Representative images of Phen Green fluorescence staining of label labile free iron in shCNMT macrophages treated with or without PQS. Phen Green: green; Hoechst (nucleus): blue. Scale bar = 20 µm. C Quantification of labile free irons in shCNMT macrophages treated in (B). Data are presented as mean ± SD (n = 3, one-way ANOVA with Tukey’s multiple comparisons test). D Representative images of C11-BODIPY fluorescence staining to label lipid ROS in shCNMT macrophages treated with or without PQS; ERA as the positive control. C11-BODIPY: green; Hoechst: blue. Scale bar = 20 µm. E Quantification of lipid ROS in shCNMT macrophages treated in (D). Data are presented as mean ± SD (n = 3, one-way ANOVA with Tukey’s multiple comparisons test). Source data are provided as a Source Data file.