Fig. 2: Narrowed proliferative cartilage layer and promotion of chondrocyte hypertrophy and mineralization associated with Dnmt1 deficiency.
From: Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes
A Immunohistochemistry for DAPI (Blue), Dnmt1 (Red) and Uhrf1 (Green) in the proliferative zone (PZ) of the proximal tibial growth plate from 1-week-old Dnmt1flox (top row) and Dnmt1ΔPrx1(bottom row) mice. B Quantification of Dnmt1 and Uhrf1 expression in the PZ of growth plate chondrocytes from Dnmt1flox (n = 6) and Dnmt1ΔPrx1(n = 6) mice. C, D Immunohistochemistry of DAPI (Blue), BrdU (Red) and (C) Dnmt1 or (D) Uhrf1 (Green) in proximal tibiae from 1-week-old mice. E Safranin O/Fast green staining of proximal tibiae from 1-week-old mice; right panels show a higher magnification of the boxed areas in the left panels, showing the PZ and Hypertrophic zone (HZ) in growth plate cartilage. F Quantification of the area of PZ and HZ in growth plate cartilage layers in proximal tibiae from Dnmt1flox (n = 7) and Dnmt1ΔPrx1 (n = 7) mice in (E). G Von Kossa staining of proximal tibiae from 1-week-old mice. H Safranin O/Fast green staining of proximal tibiae from 6-week-old mice. I µCT images of tibiae from 6-week-old mice. A, C–E, G–I Representative data from at least three individual mice are shown. All data are mean ± s.d. Significance of mean differences was assessed using unpaired two-sided t-tests. Source data are provided as a Source Data file.
