Fig. 3: Decreased extracellular matrix production and cell cycle and accelerated mineralization of Dnmt1-deficient chondrocytes.
From: Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes
A Alcian Blue staining for quantitative evaluation of cartilage matrix synthesis activity in primary cultured chondrocytes obtained from Dnmt1flox (n = 4) and Dnmt1ΔPrx1 (n = 3) mice. B Alizarin Red staining for quantitative analysis of mineralization activity of primary cultured chondrocytes obtained from Dnmt1flox (n = 3) and Dnmt1ΔPrx1 (n = 3) mice. C Expression levels of genes related to chondrogenesis, mineralization and osteoblast differentiation in Dnmt1flox (n = 3) and Dnmt1ΔPrx1 (n = 3) chondrocytes with and without BMP2 treatment. D Volcano plots of RNA-Seq data from Dnmt1flox (upper panel) and Dnmt1ΔPrx1 (lower panel) chondrocytes with and without BMP2 treatment. The plots were generated automatically using RaNA-seq, and the vertical axis displays the P values prior to adjustment. E Left panel: Venn diagram of genes that had downregulated expression during differentiation of Dnmt1flox (Light blue dashed line) and Dnmt1ΔPrx1 (Light blue solid line) chondrocytes. Right panel: Enrichment analysis of specific downregulated genes (733 genes) during differentiation of Dnmt1ΔPrx1chondrocytes shows increased expression of genes associated with the cell cycle. F Left panel: Venn diagram of genes that were upregulated during differentiation of Dnmt1flox (Pink dashed line) and Dnmt1ΔPrx1 (Pink solid line) chondrocytes. Right panel: Enrichment analysis of Dnmt1ΔPrx1-specific upregulated genes (612 genes) during differentiation of Dnmt1ΔPrx1chondrocytes shows increased expression of genes associated with the extracellular matrix. A–C Data are mean ± s.d. Significance of mean differences was assessed using unpaired t-tests or Tukey’s post hoc tests with two-tailed P values. E, F Enrichment analysis P values were calculated with Metascape. Source data are provided as a Source Data file.
