Fig. 6: E2f1 and Otx1 co-modulate minor ZGA.
From: CANTAC-seq analysis reveals E2f1 and Otx1 coordinate zygotic genome activation in Xenopus tropicalis
a A schematic view of the workflow of E2f1 and Otx1 ChIP-seq experiments. b Distribution of E2f1 and Otx1 binding sites at the promoter, gene body, and intergenic region. c Heatmaps of normalized read density of ChIP-seq (n = 2 biological replicates) for E2f1 and Otx1, as well as CANTAC-seq at five developmental stages. ChIP-peaks were categorized into three subgroups: E2f1-bound peaks (E2f1 only, n = 12,031), common peaks bound by E2f1 and Otx1 (E2f1&Otx1, n = 10,439), and Otx1-bound peaks (Otx1 only, n = 55,395). d Integrative heatmaps depicting the expression of E2f1 and Otx1 co-regulated genes (n = 220) in uninjected WT, e2f1 MO, otx1 MO, and double MO embryos at stages 7 and 8. Genes with E2f1 or Otx1 ChIP-peaks at promoter regions are marked in black. e Genome browser view of ChIP-seq, RNA-seq, and CANTAC-seq normalized read counts at sia1/2, mixer and mix1, and gsc loci.
