Fig. 6: The effect of eight MALAN mutations on NFI-X193 binding to DNA. | Nature Communications

Fig. 6: The effect of eight MALAN mutations on NFI-X193 binding to DNA.

From: Structural basis of Nuclear Factor 1-X DNA recognition provides prototypic insight into the NFI family

Fig. 6

Electrophoretic mobility shift assay (EMSA) experiments were carried out for eight (Arg38Leu, Lys113Glu, Arg115Trp, Arg116Gln, Arg116Pro, Arg121Pro, Lys125Glu, and Arg128Gln) mutations, within the NFI-X DNA-binding domain, identified in ten Malan syndrome individuals; each mutation is found in a different patient, except for Arg115Trp and Arg116Gln that were identified in two individuals each6. The effect of Malan mutations (single letter code) on DNA binding is shown in comparison with the NFI-X193 control, using 20 nM Cy5-labeled 31 bp DNA probe (sequence shown in Fig. 1) and 1X, 2X, 4X, and 8X concentrations of protein with respect to the probe. Bands corresponding to the free probe or the NFI-X193/DNA complex are indicated. All EMSAs were performed at least in triplicate with independent experiments.

Back to article page