Fig. 5: A male-like profile of WAT in female Ncoa1 KO mice, characterized by visceral fat redistribution and reduced beiging.
From: NCOA1 is a gatekeeper of the sexually dimorphic thermogenic activity of white adipose tissue
a Body weight gain of 12-month-old female (n = 8 / genotype) and male (n = 12 / genotype) Ncoa1 KO mice compared with sex-matched WT littermates. b Body weight of 12-month-old female (n = 8) and male (n = 12) WT and Ncoa1 KO mice (left) and representative image of 12-month-old female WT and KO mice (right). c MRI quantification of fat and lean mass in 12-month-old female (WT: n = 4; Ncoa1 KO: n = 4) and male mice (WT: n = 4; Ncoa1 KO: n = 5). d X-ray CT images of fat mass (orange) in 12-month-old female WT (n = 6) and Ncoa1 KO (n = 7) mice. Sagittal 3D reconstructions at L2/L4 and L5/S1 and longitudinal images from L1 to S4 are shown. e, f X-ray CT quantification of SAT and VAT in 12-month-old female WT (n = 6) and Ncoa1 KO (n = 7) mice. g, h X-ray CT quantification of SAT and VAT in 12-month-old male WT (n = 4) and Ncoa1 KO (n = 4) mice. i Hematoxylin–eosin staining of SAT from 12-month-old female WT (n = 6) and Ncoa1 KO (n = 7) mice. Arrows indicate multilocular adipocytes. Scale bar: 50 μm. j qRT-PCR analysis of Ucp1 (females: WT n = 4, Ncoa1 KO n = 6; males: WT n = 3, Ncoa1 KO n = 4) and Cidea (females: WT n = 4, Ncoa1 KO n = 6; males: WT n = 6, Ncoa1 KO n = 4) expression in SAT from 12-month-old female and male WT and Ncoa1 KO mice. k qRT-PCR analysis of Ucp1 (females: WT n = 5, Ncoa1 KO n = 6) and Cidea (females: WT n = 5, Ncoa1 KO n = 7) expression in VAT from 12-month-old female mice. l qRT-PCR in differentiated adipocytes derived from SAT SVF of 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). Values are mean ±SEM. One-way ANOVA with Tukey’s post hoc test was applied for panels a - b; unpaired two-sided t-test for panels c, e–h, and j–l. Source data provided as a Source Data file.
