Fig. 6: Glucose intolerance, liver steatosis, and impaired locomotor activity in overweight female Ncoa1 KO mice upon aging.
From: NCOA1 is a gatekeeper of the sexually dimorphic thermogenic activity of white adipose tissue
a, b Food intake measured over 3 days (white) and 3 nights (gray) in 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). c Food intake measured across 7 consecutive days in 12-month-old female WT and Ncoa1 KO mice (n = 4 per group). d Energy expenditure recorded over 24 h in 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). e, f Locomotor activity (average total distance over 24 h) in 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). White/black bars represent day/night. g Representative H&E staining of BAT from 12-month-old female WT and Ncoa1 KO mice. Scale bar: 50 μm. h qRT-PCR analysis of metabolic genes in BAT from 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). i Rectal temperature after 16 h fasting in 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). j Cold chamber test in 12-month-old female WT (n = 5) and Ncoa1 KO (n = 7) mice for 3 h. k Glucose tolerance test (GTT) showing impaired glucose tolerance in 12-month-old female Ncoa1 KO mice vs. WT (n = 6 per group). l GTT in 12-month-old male WT and Ncoa1 KO mice showing no difference (n = 6 per group). m–o Plasma glucose in (m) (WT n = 5, Ncoa1 KO n = 6), insulin in (n) (n = 6 per group), and cholesterol in (o) (n = 6 per group) in 12-month-old female WT and Ncoa1 KO mice. p Representative H&E and Oil Red O staining of liver from 12-month-old female WT and Ncoa1 KO mice, showing steatosis in Ncoa1 KO mice (n = 6 per group). Scale bar: 50 μm. q Liver triglyceride quantification in 12-month-old female WT (n = 6) and Ncoa1 KO (n = 7) mice. r qRT-PCR of lipid and cholesterol metabolism genes in liver from 12-month-old female WT and Ncoa1 KO mice (n = 6 per group). s Western blot of Akt and p-Akt (Ser473) in liver of 12-month-old female WT and Ncoa1 KO mice, with or without insulin injection (1.0 U/kg, 10 min). Two independent biological replicates were loaded for each condition on the same blot. Hsp90 served as a loading control. Data are mean ±SEM. Unpaired two-sided t-test used in panels a–c, e–f, h–o, and q, r. ns: not significant. Source data provided as a Source Data file.
