Fig. 3: The HLA-bound peptides formed by C > U mutations are immunogenic in vitro.
From: C > U mutations generate immunogenic peptides in SARS-CoV-2
A The receiver-operator characteristic (ROC) curve indicates the specificity and sensitivity of binding affinity predictions (NetMHCpan 4.0 algorithm) in determining the presence or absence of in vitro binding. Empirical binding strength values were dichotomized using an established cutoff of 45, as suggested by ProImmune Ltd. The area under the curve (AUC) is also indicated. B The fraction of CD25 + CD8 + cells in PMBCs without simulation, and after treating them either with the original or the C > U-mutated peptide pools. Each point triplet represents values for the same PBMC donor (n = 14 individuals). Two-sided Friedman test P = 8.78 × 10−6, two-sided post-hoc Conover test P-values are indicated above horizontal lines. In boxplots, horizontal lines indicate median, boxes indicate interquartile range, and vertical lines indicate first quartile – 1.5 × IQR and third quartile + 1.5 × IQR. Source data are provided in the Source Data file.
