Fig. 6: Heterogeneous responses of tonsillar and intestinal CD4⁺ T-cell subpopulations to LRAs.

Viral reactivation with LRAs in the ART-treated CD4⁺ T-cell clusters from tonsillar and intestinal tissues: a C03:T_CM, b C06:T_{TEM CD69+}, c C08:T_{FH CD69+ CCR7+}, d C09:T_{FH CD69+ CCR7-}, e C10:T_{RM CD69+ PD-1+}, f C11:T_{RM CD69+ CD49a+}, and g C12:T_{RM CD69+ CD49a+ CD103+}. Graphs displaying the percentage of p24⁺ cells within the CD4⁺ T-cell subpopulations in unstimulated (ART) and LRA-stimulated conditions: PMA and ionomycin (PMA+Iono), Ingenol (ING), Romidepsin (RMD), Ingenol and Romidepsin (I+R), Panobinostat (PNB), AZD5582 (AZD) and IL-15. Percentages of p24⁺ cells in ART were subtracted from those in each LRA-stimulated condition. N = 9 biological replicates per tissue. Medians with quartiles are represented. Statistical comparisons between unstimulated and LRA-reactivated conditions were conducted using the two-sided Wilcoxon test, with significance levels indicated numerically. All experiments were independently replicated, each run including tissue samples from distinct donors. Source data are provided as a Source Data file.