Fig. 7: HIV-reactivation of sorted CD4+ T-cell subpopulations from tonsillar and intestinal tissues. | Nature Communications

Fig. 7: HIV-reactivation of sorted CD4+ T-cell subpopulations from tonsillar and intestinal tissues.

From: Identification of inducible HIV reservoirs in tonsillar, intestinal and cervical tissue models of HIV latency

Fig. 7

Percentage of p24+ cells in sorted (a) tonsillar (TO) TNA (CD45RO⁻ CCR7⁺ CD69⁻) and TFH (CXCR5⁺ PD-1⁺) subpopulations and (b) intestinal (GUT) TRM (CD45RO⁺ CCR7⁻ CD69⁺ CXCR5⁻) and TNON-RM (CD69⁻) CD4⁺ T cells unstimulated (ART) and following stimulation with PMA and ionomycin (PMA+Iono), the combination of Ingenol and Romidepsin (I+R) and IL-15. Ingenol (ING) was also used for tonsillar cells. Percentages of p24+ cells in ART were subtracted from those in each LRA-stimulated condition. Means with standard error of means (SEM) are represented. c Effect of LRA stimulation on the expression of phenotypic markers in sorted CD4⁺ T-cell populations from tonsillar and intestinal tissues. Histograms represent the Mean Fluorescence Intensity (MFI) of each marker across conditions within the CD4+ T-cell subpopulations. N = 1 and 2 biological replicate for tonsils and intestine, respectively. Experiments with intestinal samples were duplicated in independent runs, each run including one tissue sample from a distinct donor. Source data are provided as a Source Data file.

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