Fig. 1: The screening of testis-specific mitochondrial proteins.
From: The S-depalmitoylase ABHD10 is essential for sperm mitochondrial sheath formation and male fertility
a Schematic workflow for analyzing mitochondrial proteins (MPs) across 14 murine tissues. Tissue specificity was determined by calculating the specificity measure (SPM) score for each protein, with SPM > 0.5 defining tissue-biased MPs and SPM = 1 indicating tissue-specific MPs. This panel was partly generated by Servier Medical Art and SciDraw (credit Milica Nedeljković, John Chilton and Macauley Smith Breault), licensed under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/). The drawing of testis is modified from an original provided by Servier Medical Art. b Proportional representation of mitochondrial protein populations: total MPs (cyan), tissue-biased MPs (blue), and tissue-specific MPs (purple). Tissue distribution of testis-enriched MPs among all tissue-biased (c) and tissue-specific (d) mitochondrial proteins, shown as percentage contributions. e The GO term of testis-enriched MPs. The most significantly enriched pathway was spermatogenesis. The names of the genes detected in each pathway are listed above the corresponding bars. Statistical significance (p-values) was determined based on the cumulative hypergeometric distribution. f, g RT-qPCR analyses of Abhd10 mRNA levels in tissues of mouse and human, and cell lines. n = 3 biologically independent mice. Data are presented as mean ± SEM.
