Fig. 2: QtEnc CLP tagging has minimal impact on Rubisco expression and assembly in E. coli.

a RbcL and RbcS assembly in Form I L₈S₈ RsRubisco and NtRubisco and the Form II L₂ Rr Rubisco. Structures depicted using ChimeraX with the PDB coordinates indicated. b Partial transparent surface renders of each Rubisco to view one RbcS as a ribbon structure in both Form I enzymes (their five C-terminal amino acids (C) shown in blue) and one RbcL in ribbon format in the Rr Rubisco dimer (the N-terminus (N) and C-terminus also in blue). An expanded view of the RbcS structures is provided in Supplementary Fig. 1. c Schematic of Rubisco expression plasmids with the amino acid sequence of the terminally appended Ser-Gly linker and cargo loading peptide (CLP) sequence shown. d Coomassie-stained standard native PAGE gels (8 µg protein/lane) and Rubisco antibody immunoblots (2 µg protein/lane) for analysis of cell soluble protein, enabling the quantity and mobility of each CLP-tagged (L₈S^c₈, ^cL₂, L^c₂) and untagged (L₈S₈, L₂) Rubisco to be compared. EV = pET16 empty vector control. Data shown is representative of at least two independent experiments. Source data are provided as a Source Data file.