Fig. 5: Pharmacological manipulation of seizure associated genes influences seizure responses.
From: Antecedent enhancer activity predicts future susceptibility to seizures in mice
A Paradigm for treatment of mice with HTR1F agonist LY344864 or Let-7 antagonist (antisense oligonucleotide [ASO]) and subsequent administration of PTZ to characterize seizure responses. Created in BioRender. Schoch, K. (2025) https://biorender.com/y53fyfl. B Mice were treated with HTR1F agonist or dimethyl sulfoxide (DMSO) vehicle for 3 days at P28 by daily intraperitoneal injection and responses to seizure induction were characterized. Seizure scores were plotted between control treatment versus LY344864 (n = 12,12; two-tailed Mann–Whitney p = 0.5466). Cumulative distribution demonstrating time in seconds between administration of PTZ and onset of C first response (n = 12, 12; 2-way ANOVA; p = 0.06), D score 3 (n = 11,12), E or score 5 (n = 10, 11; p = 0.02). Source data are provided as a Source Data file. F Female mice were treated with Let-7 ASO or scramble ASO control for 4 weeks from 3 months of age by intracerebroventricular injection, and responses to seizure induction were characterized. Seizure scores were plotted between scramble ASO sequence and Let-7 ASO (n = 12,12; two-tailed Mann–Whitney p = 0.0474). G–I Survival curves demonstrating time in seconds between administration of PTZ and onset of G first response (n = 12, 12; 2-way ANOVA, p = 0.003), H score 3 (n = 11, 12; p = 0.06), I or score 5 (n = 6,10). Error bars in (B, F) represent SEM.
