Fig. 4: Structural dynamics of the discriminator domain of core T-box riboswitch induced by uncharged and charged tRNA binding.
a–c Schematic representation for the respective fluorescent labeling constructs for smFRET analysis. The green and blue circles represent the Cy3 and Cy5 fluorophores, respectively. d–f Representative smFRET traces (left), FRET histograms (middle) and transition density plots (right) for each smFRET construct in (a) in four different conditions: in the absence of tRNA, in the presence of tRNAΔ, tRNAIle and tRNA78. Green, blue and black lines represent the Cy3 intensity, Cy5 intensity and FRET efficiency, respectively. FRET histograms were well fitted with Gaussian peaks, shown in red and blue for the low- and middle- or high-FRET, respectively. Transition density plots illustrate number of transition events per second. N denotes the total number of molecules used to generate the FRET histograms from three independent experiments (n = 3). Source data are provided as a Source Data file.
