Fig. 7: Recognition of tRNA NCCA end by full-length ileS T-box riboswitch is presumably co-transcriptionally controlled. | Nature Communications

Fig. 7: Recognition of tRNA NCCA end by full-length ileS T-box riboswitch is presumably co-transcriptionally controlled.

From: tRNA-dependent conformational dynamics and folding coordinate translational regulation by a full-length T-box riboswitch

Fig. 7

a Schematic representation for the secondary structure of smFRET construct used to probe the structural dynamics of AntiS/sequestrator domain. b FRET histograms for each smFRET construct in a with different lengths in the absence or presence of tRNAIle. N denotes the total number of molecules used to generate the FRET histograms from three independent experiments (n = 3). c Schematic representation for the secondary structure of smFRET construct used in co-transcriptional and post-transcriptional assay. The green and blue circles represent the Cy3 and Cy5 fluorophore, respectively. d FRET histograms for smFRET construct in (c) with different lengths prepared by post-transcriptional manner in the presence of tRNAΔ or tRNAIle. e Schematics for the sample prepared by post-transcriptional vs co-transcriptional manner used for smFRET assay. f FRET histograms for full-length T-box/tRNA complex in (c) prepared by post-transcriptional or co-transcriptional manner in the presence of tRNAΔ, tRNAIle, or tRNA78. Source data are provided as a Source Data file.

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