Fig. 3: Deficiency or inhibition of CTSL in eosinophils attenuates allergic airway inflammation in vivo. | Nature Communications

Fig. 3: Deficiency or inhibition of CTSL in eosinophils attenuates allergic airway inflammation in vivo.

From: Lysosomal acidity and cathepsin L activate eosinophils via ARG1-mediated arginine metabolism in allergic airway inflammation

Fig. 3

A–G Ctsl eoΔ mice and their littermate controls were intratracheally instilled with 100 µg of HDM on days 0, 7, and 14 (Control-NS, n = 4 biological replicates; Control-HDM, n = 5 biological replicates; Ctsl eoΔ-NS, n = 4 biological replicates; Ctsl eoΔ-HDM, n = 6 biological replicates). H–N C57BL/6 mice were intratracheally instilled with 100 µg of HDM on days 0, 7, and 14. SID26681509 was administered via intraperitoneal injection at a dose of 10 mg/kg on days 7, 9, 11, 13, and 15 (Control-NS, n = 5 biological replicates; Control-HDM, n = 5 biological replicates; SID26681509-NS, n = 4 biological replicates; SID26681509-HDM, n = 6 biological replicates). A, H The count of inflammatory cells in BAL fluid from mice. B, I The mRNA levels of Il4, Il5, and Il13 in lung tissues analyzed by RT-qPCR. C, J Representative Hematoxylin and Eosin (H&E) stained lung tissue sections from mice. Scale bars, 200 µm. D, K Histological inflammatory scores from H&E images. E, L Representative periodic acid-Schiff (PAS)-stained lung tissue sections from mice. Scale bars, 100 µm. F, M PAS scores. G, N Airway hyperresponsiveness (AHR) in response to methacholine. Data are shown as mean ± SEM. Statistical analyses were calculated using two-way ANOVA with Sidak’s multiple comparisons test.

Back to article page