Fig. 5: ARG1 promotes eosinophil activation in allergic airway inflammation in vitro and in vivo. | Nature Communications

Fig. 5: ARG1 promotes eosinophil activation in allergic airway inflammation in vitro and in vivo.

From: Lysosomal acidity and cathepsin L activate eosinophils via ARG1-mediated arginine metabolism in allergic airway inflammation

Fig. 5

A–E Eosinophils were isolated from the peripheral blood of Il5 Tg mice and treated with or without IL33 (20 ng/ml) for 8 h. A Arginase activity in eosinophils with or without 20 µM nor-NOHA and 20 µM BEC (n = 3 biological replicates). B, C ELISA analysis (n = 3 biological replicates) of culture supernatants from eosinophils incubated with or without 20 µM nor-NOHA and 20 µM BEC for IL4 (B) and IL13 (C). D, E Flow cytometry (n = 3 biological replicates) analysis for CD69 (D) and ICAM-1 (E) expression on eosinophils with or without 20 µM nor-NOHA and 20 µM BEC. F–K C57BL/6 mice were intratracheally instilled with 100 µg of HDM on days 0, 7, and 14. Saline or nor-NOHA was administered via intratracheal instillation at a dose of 4 mg/kg on days 14, 15, and 16 (Control-NS, n = 3 biological replicates; Control-HDM, n = 6 biological replicates; nor-NOHA-NS, n = 4 biological replicates; nor-NOHA-HDM, n = 6 biological replicates). F The count of inflammatory cells in BAL fluid from mice. G The mRNA levels of Il4, Il5, and Il13 in lung tissues analyzed by RT-qPCR. H Representative H&E stained lung tissue sections from mice. Scale bars, 200 µm. I Histological inflammatory scores from H&E images. J Representative PAS-stained lung tissue sections from mice. Scale bars, 100 µm. K PAS scores. Data presented are representative of three independent experiments shown as mean ± SEM. Statistical analyses were calculated using two-way ANOVA with Sidak’s multiple comparisons test.

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