Fig. 6: CTSL promotes eosinophil activation by enhancing arginine metabolism via ARG1. | Nature Communications

Fig. 6: CTSL promotes eosinophil activation by enhancing arginine metabolism via ARG1.

From: Lysosomal acidity and cathepsin L activate eosinophils via ARG1-mediated arginine metabolism in allergic airway inflammation

Fig. 6

A, B, D–G Eosinophils were isolated from the peripheral blood of Il5 Tg mice and treated with or without IL33 (20 ng/ml) and 50 µM SID26681509 for 8 h. A ELISA analysis (n = 3 biological replicates) of culture supernatants from eosinophils with or without ARG1 overexpression for IL4 and IL13. B ELISA analysis (n = 3 biological replicates) of culture supernatants from eosinophils in the presence or absence of ARG1 protein (2 µg/ml) and 20 µM nor-NOHA for IL4 and IL13. C Eosinophils were isolated from the peripheral blood of control and Ctsl eoΔ Il5 Tg mice. ELISA analysis (n = 3 biological replicates) of culture supernatants from eosinophils in the presence or absence of ARG1 protein. D, E Concentrations of arginine and ornithine in eosinophils (n = 5 biological replicates). F, G ELISA analysis (n = 3 biological replicates) of culture supernatants from eosinophils in the presence or absence of ornithine (5 mM). Data presented are representative of three independent experiments and shown as mean ± SEM. Statistical analyses were calculated using two-way ANOVA with Sidak’s multiple comparisons test.

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