Fig. 7: Lysosomal function and ARG1 increases in activated eosinophils, and lysosomal acidity, CTSL activity correlate with disease severity in humans. | Nature Communications

Fig. 7: Lysosomal function and ARG1 increases in activated eosinophils, and lysosomal acidity, CTSL activity correlate with disease severity in humans.

From: Lysosomal acidity and cathepsin L activate eosinophils via ARG1-mediated arginine metabolism in allergic airway inflammation

Fig. 7

A–I Granulocytes were isolated from the peripheral blood of healthy volunteers (n = 13) and asthmatic patients (n = 23). Lysosome-associated membrane protein (LAMP) 1 (A) and LAMP2 (B) expression, lysosomes acidity (C), CTSL enzyme activity (D), and ARG1 expression (E) in eosinophils. Spearman correlations between lysosomal acidity and forced expiratory volume in one second (FEV1) % pred (F), and FEV1/ forced vital capacity (FVC) (%) (G). Spearman correlations between CTSL enzyme activity and FEV1 % pred (H), and FEV1/FVC (%) (I). Data presented are shown as mean ± SEM (A–E). Statistical analyses were calculated using two-sided unpaired Student’s t test. Data are combined from two independent cohorts of asthmatic patients and healthy controls, with each individual sample representing a distinct biological replicate.

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