Fig. 4: Identification of patient-specific drugs through drug screening using the EoC-ERS2. | Nature Communications

Fig. 4: Identification of patient-specific drugs through drug screening using the EoC-ERS2.

From: Microengineered patient-derived endometrium-on-a-chip for the evaluation of endometrial receptivity and personalised translational medicine

Fig. 4

a Schematic of experimental design patterning on the synechia-EoC, with isolated patient-derived cells cultured to replicate the patient’s endometrial microenvironment for drug screening, and the development of the ERS2 for evaluation. Created in BioRender. Ahn, J. (2025) https://BioRender.com/d88wxaub Immunofluorescence (IF) analyses of integrin αvβ3 (red) and OPN (green) with DAPI (Blue) in patient-derived EoCs (normal vs. synechia- NT, +CXCL12, +hCG, or +VitD3) compared to mouse synechia-modelled endometrial tissues under same treatment conditions. Scale bar; 50 µm. Quantification of integrin αvβ3 (c) and OPN (d) IF intensity in the EoCs (c, d) and corresponding mouse endometrial tissues (e, f) (c; Normal vs. NT: p = 0.0293, NT vs. +hCG: p = 0.0004, +CXCL12 vs. +hCG: p = 0.012, +hCG vs. +VitD3: p = 0.0206/ e; Normal vs. NT: p = 0.0024, Normal vs. +CXCL12: p = 0.011, Normal vs. +VitD3: p = 0.0268, NT vs. +hCG: p = 0.012/ f; Normal vs. NT: p = 0.0238, NT vs. +hCG: p = 0.0253) n = 3 independent patient-derived EoCs; n = 3 independent mice. g IF staining images of blood vessels (CD31; red) in patient-derived EoCs (normal vs. synechia- NT, +CXCL12, +hCG, or +VitD3), compared to mouse endometrial tissues under same treatment conditions. Scale bar: 50 µm. Quantification of areas of angiogenic sprout (h) and blood vessels (i) in the EoCs, and blood vessel intensity (j) and blood vessel number (k) in corresponding mouse endometrial tissues (h; NT vs. +CXCL12: p < 0.0001, NT vs. +VitD3: p = 0.0001, +CXCL12 vs. +hCG: p < 0.0001, +hCG vs. +VitD3: p = 0.0001/ i; Normal vs. NT: p = 0.0007, NT vs. +CXCL12: p < 0.0001, NT vs. +VitD3: p < 0.0001, +CXCL12 vs. +hCG: p = 0.0006/ j; NT vs +CXCL12: p = 0.0018, k; Normal vs NT: p = 0.0219, NT vs. +CXCL12: p = 0.0007, NT vs. +hCG: p = 0.0295, NT vs. VitD3: p = 0.0442). n = 3 independent patient-derived EoCs; n = 3 independent mice. Data shown as mean ± SD and analysed by one-way ANOVA including P-values (*p < 0.05, **p < 0.01, *** p < 0.001, ****p < 0.0001.) l IF staining of spheroids loaded into the EoCs (normal vs. synechia- NT or +CXCL12), showing Jeg3 (red), F-actin (green), and DAPI (blue). Scale bar: 50 µm. m Quantification of depth of trophoblast invasion (12 h; Normal vs NT: p < 0.0001, NT vs. +CXCL12: p < 0.0001/ 24 h; Normal vs. NT: p = 0.0103, NT vs. +CXCL12: p = 0.0337/ 48 h; Normal vs. NT: p = 0.0119, NT vs. +CXCL12: p = 0.0104). n = 3 independent experiments using spheroids. Data shown as mean ± SD and analysed by two-way ANOVA including P-values (*p < 0.05, **p < 0.01, *** p < 0.001, ****p < 0.0001). Source data are provided as a Source Data file.

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