Fig. 1: scRNA-seq of esophageal eosinophils.
From: Epigenetic and transcriptional programming of murine eosinophils in the esophagus
A UMAP of scRNA-seq esophageal cell populations with population annotations. B Heatmap showing gene expression levels normalized per row of identified hallmark gene expression for esophageal resident cell populations. C Violin plot showing the number of genes, number of UMI’s and percentage mitochondrial genes per cluster in the esophageal scRNA-seq dataset. D PlotProfile showing two esophageal eosinophil populations (Eo1, Eo2) with gene expression of eosinophil marker genes. Plots are colored by the relative expression of gene transcripts. E Radar map showing top gene ontology terms enriched in esophageal eosinophils compared to neutrophils and mast cells, emphasizing metabolic, extracellular sensing, and immunomodulatory gene pathways. F Heatmap showing normalized gene expression values for select genes enriched in the murine esophageal eosinophil scRNA-seq dataset. Gene expression is normalized within each row. G PlotProfile showing expression of eosinophil granule protein genes and early activation marker genes in esophageal eosinophil populations present in the murine esophagus. Plots are colored by the relative expression of gene transcripts. H Left; PlotProfile of C3ar1 gene expression. Right; Quantification of esophageal eosinophil surface expression from CC10-iIL13Tg mice (isotype: n = 3, + C3aR1 antibody: n = 14, 3 independent experiments) with, Bottom; representative histogram compared to isotype control. Scatter plots show the mean with standard deviation error bars. Statistical analysis shows a two-sided paired T test. I Heatmap showing expression (gMFI) of eosinophil markers between tissue locations. Expression is normalized per row. J Bar chart, with representative histograms, showing eosinophil expression of C3aR1 from diverse tissue locations (bone [blue], blood [red], esophagus (esoph. [orange]), lung [pink], duodenum (duo. [light green]), and ileum [dark green]) in CD2-IL5Tg mice (blood, bone and esophagus: n = 8, lung, duodenum and ileum: n = 7, 2 independent experiments). Scatter plots show the mean with standard deviation error bars. Statistical analysis shows one-way ANOVA with Tukey’s multiple comparisons. *** p < 0.0001 compared to blood. ### p < 0.0001 compared to bone marrow. ## p = 0.0076 compared to bone marrow.
