Fig. 2: Epigenetic landscape of esophageal eosinophils.

A Heatmap showing peak score intensity of differentially bound regions of open chromatin from bone marrow and esophageal eosinophils as determined by ATAC-seq (red box: esophageal eosinophils, dark blue box: mature bone marrow eosinophils, light blue box: immature bone marrow eosinophils). B Volcano plot showing regions with differential chromatin accessibility with FDR < 0.05, Fold Change ≥ 2, as determined by DESeq2. Accessible regions of chromatin are annotated with key genes based on the closest location. Note that the y-axis scale has been log-transformed. C Ranked gene ontology plot displaying enrichment score (Fisher exact test) for key gene pathways expressed as log10(p-value) in esophageal (red-ringed circle) and mature bone marrow (blue-ringed triangle) enriched accessible regions of chromatin. Gene ratio, the proportion of genes in the described gene ontology (GO) term which are enriched in our gene list. D Genome profile view showing chromatin accessibility surrounding differentially regulated regions of the eosinophil epigenome. Genes shown; C3ar1, Cd80, Retnla and Mpo. The histogram shows gene expression levels (data taken from GEO: GSE81135²) as mean ± SD; individual markers represent gene expression in individual mice (n = 3). Statistical analysis shows a two-sided Student t test. ESOPH, esophagus; BM, bone marrow.