Fig. 5: Esophageal eosinophil levels in ATF3-KOeoCre mice.

A Allergen-induced esophageal eosinophilia model. Calci., calcipotriol; Alt., Alternaria alternata; EtOH, ethanol; iso., isotype control; SigF, SIGLEC-F; DCs, dendritic cells. B Proportion (of CD45 + cells) of esophageal eosinophils (WT PBS: n = 9, ATF3-KOeoCre PBS: n = 10, WT Allergen challenged: n = 15, ATF3-KOeoCre Allergen challenged: n = 16, 3 independent experiments). Scatter plots show the mean with standard deviation error bars. Statistical analysis shows two-way ANOVA with Tukey’s multiple comparisons. C UMAP of scRNA-seq esophageal cell populations with population annotations. D Heatmap showing gene expression levels normalized per row of identified hallmark gene expression for esophageal resident cell populations. E Volcano plot of global pseudobulk analysis (DESeq2; log2FC = 0.1, pdaj < 0.05) between WT allergen-treated and ATF3-KOeoCre allergen-treated esophageal scRNA-seq datasets. F Esophageal eosinophil sub-clustering on WT allergen-treated and ATF3-KOeoCre allergen-treated samples. G Heatmap showing normalized expression of the 44 genes differentially regulated between ATF3-KOeoCre allergen-treated esophageal eosinophils and WT allergen-treated esophageal eosinophils, which overlap with the esophageal eosinophil epitranscriptome. Of these, only one - Crtc3 (annotated with asterix) - was significantly different in PBS- treated WT and ATF3-KOeoCre mice. H Histogram showing esophageal eosinophil expression levels of C3aR1 between genotypes compared to isotype control (blue), WT: black, ATF3-KO: red, with quantification (isotype: n = 3, WT: n = 15, ATF3-KO: n = 16, 3 independent experiments). Data are presented as mean ± SD; individual markers represent an individual mouse. Statistical analysis shows one-way ANOVA with Tukey’s multiple comparisons.