Fig. 5: Cell deformation triggers Ca2+ wave propagation mediated by MCCs. | Nature Communications

Fig. 5: Cell deformation triggers Ca2+ wave propagation mediated by MCCs.

From: Mechanochemical mechanism underlying intercellular Ca2+ wave propagation and its crucial role in apoptotic cell extrusion

Fig. 5

a Temporal dynamics of Ca²⁺ transients at representative junctions. To induce cell deformation without triggering apoptosis, a femtosecond laser at 10 nJ/pulse was focused on the site marked with a red asterisk through a 100×/1.25 NA objective lens. Control (DNSO, top panel), GsMTx4 (upper panel), CBX (lower panel), and trpc1-/- plus piezo1-MO (bottom panel). The right panels show kymographs of the white-boxed regions on the left. Scale bar: 20 µm. b The normalized GCaMP7 intensity of the apoptotic cell and adjacent surrounding cells, and displacement of the contact edge. Control (DMSO, top graph), GsMTx4 (upper graph), CBX (lower graph), and trpc1-/- plus piezo1-MO (bottom graph). Arrows indicate time points of laser irradiation. The shaded region represents the standard error of the mean. Control: n = 14 embryos, CBX: n = 8 embryos, GsMTx4: n = 8 embryos, and trpc1-/- plus piezo1-MO: n = 10 embryos.

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