Fig. 5: The serotonin receptor SER-7 drives aversion from the mediocre ∆crp diet.

a Behavioral responses of wild-type, tph-1, and serotonin receptor mutant animals on the mediocre ∆crp diet. Aversion is diminished in two ser-7 null mutants and in mod-1 and lgc-50 mutants (n = 8–24 assays). The ser-7(tm1325) allele was used in all subsequent experiments. b Aversion behavior of ser-7 mutants expressing ser-7 cDNA from its endogenous promoter (ser-7), or a promoter for overlapping neurons (flp-21). Two transgenic lines were tested for each plasmid (n = 9–27 assays). c Aversion behavior of ser-7 mutants with ser-7 rescue in the I1 neuron and other sets of enteric neurons. Two transgenic lines were tested for each rescue plasmid (n = 9–73 assays). d Schematic model of SER-7 serotonin signaling in aversion from the mediocre ∆crp diet. For (a–c), each data point indicates individual assay. Results are shown with median ± quartiles in boxes and Min to Max whiskers. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA corrected by Dunnett’s (a, b) or Tukey’s (c) multiple comparisons. Full genotypes of all C. elegans strains are provided in Supplementary Data 3. All sample sizes, statistical tests used, and exact P values are provided in Supplementary Data 4. Source data are provided as a Source Data file.