Fig. 2: PUMA and BCL-xL tail anchors cooperate to limit BH3 antagonism. | Nature Communications

Fig. 2: PUMA and BCL-xL tail anchors cooperate to limit BH3 antagonism.

From: Allosteric regulation of BH3-in-groove interactions by tail anchors of BCL-xL complexes limits BH3 mimetic antagonism

Fig. 2

a Schematic representation of different protein variants expressed in the cells for this study. BH3 domain of initiators and TA are indicated. The numbering indicates the amino acid position in the full-length protein. The pictograms used to describe the complexes evaluated in the BRET dose-responses analysis are displayed on the right. See Supplementary Table 1 for sequences. b Dose-response antagonism of YBCL-xL by WEHI-539, depending on the interaction with RPUMA-BH3BID variants in MCF-7 cells. The representation is as described in Fig. 1b. c Dose-response antagonism of YBCL-xL by A-1331852, depending on the interaction with RPUMA variants in MCF-7 cells. Note that for ease of comparison, the data for the response of the RPUMA/YBCL-xL complex shown in Fig. 1b have been reproduced. The representation is as described in Fig. 1b. d Dose-response antagonism of YBCL-xL and YBCL-xLdC by WEHI-539 interacting with RBH3 domains of initiators in MCF-7 cells. Note that for ease of comparison, the data for the responses of the RBH3 domain/YBCL-xL complexes shown in Fig. 1b have been reproduced. The representation is as described in Fig. 1b.

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