Fig. 5: Ingenuity pathway analysis of the effect of methylene blue treatment on gene expression in the brainstem.
From: Methylene blue treatment of fatal cerebral malaria and identification of potential blood biomarkers
a Top 20 pathways enriched in the 574 DEGs with length of the bars indicating statistical significance (p-value) of enrichment (unadjusted p-value from a right-tailed Fisher’s exact test performed using IPA software) and color shade representing IPA prediction of up or down regulation of the pathway (z-score > 0 for upregulation, z-score <0 for down regulation, z-score = 0 for no change, z-score = NA if the direction of regulation cannot be predicted). b Pathways found to be overlapping and forming the most significant pathway cluster by IPA. c Representation of the neuroinflammation signaling pathway based on the list of 574 DEGs modulated by both infection and MB treatment in the brainstem. Data were analyzed with the use of QIAGEN IPA (QIAGEN Inc., https://digitalinsights.qiagen.com/IPA)98.
