Fig. 6: Siglec-Fhi neutrophils enhance the differentiation of Th17 cells.
a Volcano plot displaying differential gene expression between Siglec-Flo and Siglec-Fhi neutrophils (FDR < 0.05, absolute logFC > 2 highlighted). b Gene set enrichment analysis of differentially expressed genes. c, d UMAP plots (c) and violin plots (d) for cytokine genes related to Th17 differentiation. e Correlation between the proportion of Siglec-Fhi neutrophils and RORγt+ or IL-17A+ CD4 T cells in asthmatic lungs. EpxCre/+ (n = 14), EpxCre/+Gpr43fl/fl (n = 15). Data were pooled from three independent experiments. f Experimental scheme for co-culture of neutrophils and naïve CD4 T cells under Th17 differentiation conditions. Created in BioRender. Yu, J. (2025) https://BioRender.com/j8a0ji3. Siglec-Flo and Siglec-Fhi neutrophils were prepared by culturing bone marrow neutrophils in the absence or presence of IL-4 and GM-CSF, respectively. g Percentages of RORγt+ and IL-17A+ CD4 T cells after the co-culture. Siglec-Flo neutrophils (n = 4), Siglec-Fhi neutrophils (n = 4). Data are representative of three independent experiments. Data are presented as mean ± s.e.m. Statistical analysis was performed using a two-sided permutation test with false discovery rate (FDR) correction for multiple comparison (b), the Wilcoxon rank-sum test with Benjamini–Hochberg correction (d), simple linear regression (e), and one-way ANOVA with Tukey’s multiple comparison test (g). ns, not significant.
