Fig. 3: Spatiotemporal change in pH of the LIDA system.
From: A chemoenzymatic reaction-diffusion system with local inhibition and distal activation
a Schematic of the reaction set up with components as described in Fig. 1c. In this case, pH probe Bromothymol blue (0.02 mM) was added in the gel instead of NBI. At the empty circular zone (5 mm diameter) aqueous solution of (ATP + urea) was added and allowed to diffuse to the agarose gel matrix (0.75 wt%). b A snapshot of petridish taken after 60 min, showing the initial zone is acidic (yellow color) and outer region is basic (blue color). c Spatiotemporal pattern of pH in the gel matrix with time when 40 µl of aqueous solution of 5 mM ATP and 50 mM urea was injected in the central zone. Blue indicates pH of 8 or higher and red indicates pH lower than 6. The pH of the whole gel before injection was around 5.6 as it was made with mQ water (which is slightly acidic due to dissolved CO2). d pH (blue points, left sided Y-axis) and product formation (orange points, right-sided Y-axis) with time at zone 1, zone 3 and zone 5. Data are presented as mean ± SD (where n = 8). Source data are provided as a source data file.
