Fig. 4: Numerical simulation to show spatiotemporal pattern of product formation in the reaction-diffusion based chemoenzymatic system.
From: A chemoenzymatic reaction-diffusion system with local inhibition and distal activation
a Scheme of the reactions used for modeling. Here similar dimensions of two-dimensional circular disc (8 mm diameter) as used in experiment were chosen. b Spatiotemporal distribution of reaction product (P) at different kIN/kA and different diffusion coefficient ratios of ProA, IN and A when ProA (50 mM) and IN (5 mM) are injected at the center (left) and A (50 mM) and IN (5 mM) are injected at the center (right). The maximum product concentration (normalized) obtained in both ProA + IN and A + IN cases are given in Supplementary Table 1. Product distribution when kIN/kA = 103, and DProA:DIN:DA = 40: 1: 1, 40: 2: 2, 40: 5: 5 and 40: 8: 8 (where DProA = 4 × 10−10 m2/s) for c ProA + IN injection and d A + IN injection at the center at 150 min. Notably, product concentration in the case of A + IN injection is higher than ProA + IN injection. For (b–d), the concentration of product was normalized with respect to the maximum product concentration obtained for the case of kIN/kA = 103 and DProA:DIN:DA = 40:2:2 at 150 min.
