Fig. 1: Identification of candidate metabolic enzymes closely associated with inflammatory macrophage infiltration in DWs.

A Score plot depicting the separation of metabolic gene patterns in the human DW and NDW groups through PCA analysis in GSE154556. B Volcano plot showing the differentially expressed metabolic genes between human DWs and NDWs in GSE154556. Differentially expressed genes were assessed with the limma moderated two-sided t test. C KEGG analysis of typical differential metabolic pathways between human DWs and NDWs in GSE154556. D t-SNE plots of the characterized cell clusters identified via scRNA-seq of human wound samples (GSE165816). E Venn diagram showing the shared altered metabolic differential genes and their origins. F Correlation analysis of the expression levels of the metabolic candidates and the inflammatory macrophage infiltration score in GSE154556. The text annotations above showed the cellular origins of the main differences of these candidates analyzed from GSE165816. G Cellular communication analysis revealing potential interactions among pericytes with low MAT2A expression and other cell types from GSE165816. H Schematic illustration of the methionine cycle, and the relative levels of methionine in the human DW and NDW groups. n = 12 biologically independent samples. I Expression levels of metabolic enzymes involved in the methionine cycle in the two groups (GSE165816). Non-parametric two-sided Wilcoxon rank-sum test was used. J Immunofluorescence staining and statistical analysis demonstrating the expression levels of MAT2A in CD31-NG2+PDGFRβ+ pericytes from human wounds. n = 3 biologically independent samples. K Pericytes were classified into samples with high MAT2A expression levels and samples with low MAT2A expression levels (GSE165816); grouped samples were analyzed via GSEA. The median expression of the gene was used as the dividing line. Data were shown as mean ± SD. Statistical significance was determined using hypergeometric test (C) and two-tailed unpaired t test (H, J). Source data are provided as a Source Data file.