Fig. 3: Staphopain A cleaves and activates GSDMA.

a SDS–PAGE of recombinant human GSDMA cleavage by purified ScpA in the presence or absence of E-64 after 1 h. Blot is representative of three independent experiments. b Binding of GSDMA with or without ScpA to liposomes consisting of a mixture of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and cardiolipin (CL) analyzed by SDS–PAGE. The gold arrow identifies GSDMA-N. Blot is representative of three independent experiments. c Summary of experimental results in (b). d Liposome leakage monitored by sulforhodamine B (SRB) fluorescence on incubation with GSDMA with or without ScpA. Detergent added after 21 min (dotted line). Data are representative of three independent experiments and are presented as mean ± SD of biological replicates, n = 4. e SDS–PAGE of recombinant human GSDMA cleavage by ScpA over time in the presence or absence of liposomes made from PC, PE, and CL. The gold arrow identifies GSDMA-N. Summary of experimental results shown above SDS–PAGE. Blot is representative of three independent experiments. f Cleavage site on GSDMA identified by Edman sequencing. c Created in BioRender. LaRock, D. (2025) https://BioRender.com/0w1s8az (e) Created in BioRender. LaRock, D. (2025) https://BioRender.com/zgdj89u.