Fig. 4: GSDMA protects mice in S. aureus skin infection.
From: Staphylococcus aureus induces Gasdermin A-dependent keratinocyte pyroptosis
a Human GSDMA gene arrangement compared to mouse Gsdma1–3. Expression of mouse Gsdma in keratinocytes is indicated. b Lysates of HEK293T cells transfected with the indicated Flag-GSDMs were incubated with purified ScpA for 1 h and analyzed for immunoblotting for Flag-GSDMs. Data are representative of three independent repeats. Wildtype (C57BL/6 J–dark purple) and GSDMA-deficient (gsdma123−/−–light purple) mice after epicutaneous application of 108 CFUs of S. aureus and c transepithelial water loss (TEWL) monitored, and skin imaged at day 7. Results represent mean ± SD, n = 6 for each point. d At day 2 and 7 of infection, skin was examined by Hematoxylin and Eosin stain. Scale bar is 500 μM. e Colony-forming units (CFUs) of S. aureus at the site of infection were measured by plating 2 days post-infection (n = 9, WT, n = 8, ΔscpAB) and 7 days post-infection (n = 17). Results represent mean ± SD with individual biological replicates shown; one-way ANOVA with Dunnett’s multiple comparison test was used to determine statistical significance.
