Fig. 4: Elevated TOR-S6K-mediated protein synthesis leads to defects in fusome branching and oocyte determination in the Acc-depleted germline.
From: Acetyl-CoA carboxylase maintains energetic balance for functional oogenesis
A Germaria of indicated genotypes with or without rapamycin treatment (rapa) were labeled with LamC (red, cap cell nuclear envelopes) and 1B1 (red, fusomes and follicle cell membranes) or α-spectrin (red, fusomes and follicle cell membranes). Yellow lines indicate GSCs and yellow dashed circles outline germ cell cysts with unbranched fusomes. B, C Number of GSCs in the germaria in the indicated genotypes. The left y axis indicates the proportion of germaria with the indicated GSC number. The right y axis shows the average GSC number per germarium. Error bars indicate mean ± SEM. D Percentage of germ cell cysts (carrying at least 4 germ cells) in the indicated genotypes carrying single-branched or unbranched fusomes. Statistical analysis was performed using two-tailed chi-squared test. Orange-filled and-unfilled circles on the x axis in (B–D) indicate the presence or absence of the indicated RNAi. The number of germaria analyzed in B and C and germ cell cysts analyzed in D are shown above each bar. Yellow background indicates the flies were treated with rapamycin. *, P < 0.05; **, P < 0.01; ***, P < 0.001. NS, not significant. Black asterisks in B and C indicate significant differences compared with control, in D compared with Acc-KD group, or comparisons between two groups as indicated by a line. Red asterisks in C indicate significant differences compared to ACC-KD group. Statistical analysis in (B, C) were performed using the unpaired two-tailed Student’s t-test, and in D by the chi-squared test. E Ovarioles of the indicated genotypes with α-spectrin (red, fusomes and follicle cell membranes) and Orb (green, oocyte marker). Yellow asterisks indicate posteriorly located oocyte in the egg chamber, denoted as normal localization. The percentage shows the proportion of egg chambers with normal localization, and the number of egg chambers analyzed is shown in parentheses. Scale bars, 10 μm in A, 20 μm in E. RNAi expression was driven by nos-GAL4. D7, 7-day-old.
