Fig. 5: Germline depletion of Acc disrupts global endosomal trafficking at least in part through the elevated FAO-TOR-S6K-protein synthesis axis.
From: Acetyl-CoA carboxylase maintains energetic balance for functional oogenesis
A Germaria of the indicated genotypes with α-spectrin (red, fusomes and follicle cell membranes) and Rab7 (green, late endosomes). B Control and ACC-knockdown (KD) germaria expressing Lamp1-GFP (green, lysosomes) were labeled with LamC (red), 1B1 (red). Yellow dashed circles in A outline germ cell cysts with unbranched fusomes. The percentages shown indicate the proportion of germaria displaying large punctate Rab7 (A) and Lamp1-GFP (B) signals, respectively; the number of germaria analyzed is provided in parentheses. C Control and ACC-KD germaria were labeled with α-spectrin (blue, fusomes), Rab11 (green, recycling endosomes), and E-cadherin (E-cad, red). Enlarged views of the square in germaria are shown to the right with or without the Rab11 channel. Yellow lines indicate GSCs; arrows indicate the presence of E-cad in Rab11-labeled endosomes. D Pearson’s correlation coefficient values for E-cad and Rab11 colocalization in the germarium in the indicated genotypes. Each dot represents a germarium. Orange-filled and-unfilled circles on the x axis indicate the presence of S6KRNAi and gfpRNAi, respectively. Error bars indicate the mean ± SD. **, P < 0.01; ***, P < 0.001. NS, not significant. Asterisks indicate significant differences compared with the control, or comparisons between two groups indicated by a line. Statistical analyses were performed using the unpaired two-tailed Student’s t-test. Scale bars, 10 μm. UAS-RNAi expression was driven by nos-GAL4. D7, 7-day-old.
