Fig. 5: Genome-wide specificity of enDelIscB and enDelIscB-T5E. | Nature Communications

Fig. 5: Genome-wide specificity of enDelIscB and enDelIscB-T5E.

From: Engineering a CRISPR-associated IscB system for developing miniature genome-editing tools in human cells and mouse embryos

Fig. 5: Genome-wide specificity of enDelIscB and enDelIscB-T5E.

a–d Off-target editing events induced by enDelIscB and enDelIscB-T5E in HEK293T cells at AAVS1-sg3 (a), EMX1-sg4 (b), AAVS1-sg2 (c), and VEGFA-sg2 (d). A 19-nt sequence that includes TAM (3 nt) and the guide sequence (16 nt) is shown (percentiles of reads >1% are listed). e Summary of off-target site hits and the proportion of off-target editing events versus total edits.

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