Fig. 7: enDelIscB and enDelIscB-T5E-mediated efficient genome editing in mouse embryos. | Nature Communications

Fig. 7: enDelIscB and enDelIscB-T5E-mediated efficient genome editing in mouse embryos.

From: Engineering a CRISPR-associated IscB system for developing miniature genome-editing tools in human cells and mouse embryos

Fig. 7: enDelIscB and enDelIscB-T5E-mediated efficient genome editing in mouse embryos.

a Schematic representation of the mouse tyrosinase (Tyr) gene, which contains five exons. We choose exon1 and exon4 for screening target sequences. Each target sequence is annotated below its corresponding exon. b enDelIscB-T5E-mediated indel formation efficiency at different targets of Tyr gene in mouse N2a cells. Data represent mean ± s.d. of three independent biological replicates. c Phenotypic photographs of F0-generation mice with Tyr gene disruption generated by microinjecting enDelIscB or enDelIscB-T5E mRNA and sgRNA (Tyr-sg12). Photos were taken when the mice were 21 days old. d The indel rates of the Tyr gene in F0 mice were edited by enDelIscB (n = 39) and enDelIscB-T5E (n = 47). e Summary of the condition in constructing a mouse model of albinism through microinjection. Source data are provided as a Source data file.

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