Fig. 5: Reversible isomerization with green (GR) and ultraviolet (UV) stimulation.
From: Interferometric imaging of the reversible rhodopsin dynamics in the living rat eye
A Control experiment: ORG responses elicited by two green flashes (1 ms, 340 μJ measured in front of the cornea, corresponding to 3.6 × 108 photons/μm2 on the retina) delivered 200 ms apart. B Response to a flash sequence of green-UV-green-UV, with a UV flash of 1 ms, 112 μJ. Given a transmittance of 0.54 through the anterior segment of the eye at 385 nm33,34 and an illumination area on the retina of 1.56 mm2, this corresponds to approximately 7.5 × 107 photons/μm2 incident on the retina. Insets show the first 10-ms temporal window. Red arrows indicate the amplitudes plotted in (C–E). C The first elongation amplitude as a function of UV pulse energy. D The second contraction amplitude as a function of UV pulse energy. E The second elongation amplitude as a function of UV pulse energy. Dots in (C–E) show measurements in individual rats (n = 5), and error bars represent one standard deviation (SD) around the average. F ORG responses to two UV pulses (3 ms, 336 μJ, 200-ms apart, n = 5). Insets show the first 30-ms temporal window.
