Fig. 1: High-resolution in situ structure of the human ribosome.
From: Visualizing the translation landscape in human cells at high resolution
a Schematic of the in situ single-particle cryo-EM pipeline. b Consensus map of human 80S ribosome at 2.2 Å resolution (left) and the local resolution map (right). c Selected features of the 80S ribosome structure. The cryo-EM map is shown as a semitransparent surface with the fitted model in stick representation. rRNA modifications are marked with arrows. d Well-resolved cryo-EM map of the P site tRNA (P-tRNA). e Cryo-EM density of mRNA (a polyU model, blue sticks) in A, P, and E sites of the 40S subunit (bottom) and its interaction with the P-tRNA (right inset). The well-resolved density for mRNA is an average of all translating mRNAs. A schematic of the interactions is shown at the top. f Density for the nascent peptide (red) in the 60S subunit. The ribosome is shown as a gray surface clipped at the nascent peptide exit tunnel. g Cryo-EM densities of homoharringtonine (HHT) and cycloheximide (CHX) (shown as sticks) resolved in cells treated with both drugs.
