Fig. 5: Representativeness of the 7 body liquids regarding metastatic sites.

Violin plots describing the distribution of the Z-scores derived from the phylogenetic reconstructions of the 20 patients when considering all pairs of samples involving one body liquid at a time according to all other sample types (one panel per body liquid). Sample types are ordered by increasing median Z-score levels. A dashed line at 0 indicates the cutoff to distinguish between relative short (Z-score <0) and long distance (Z-score>=0). On the right, number of metastatic sites and body liquid falling in these two categories are reported below and above the line respectively. Only samples taken at autopsy are considered, samples from the lung are grouped irrespectively of their lobe of origin, and samples from the liver are grouped irrespectively of their segment of origin. In case of multiple samples available for the same body liquid in a patient, the one having the highest purity is chosen. The distribution is shown for peripheral blood (A, 369 samples), central blood (B, 292 samples), ascites (C, 387 samples), pleural fluid (D, 374 samples), CSF (E, 263 samples), pericardial fluid (F, 171 samples), and urine (G, 61 samples). In all boxplots the box delineates Q1, median and Q3, while the whisker ranges from Q1-1.5*IQR to Q3+1.5*IQR. Samples are biological replicates. Data are derived from the lpWGS. Source data are provided as a Source Data file. ASC ascites, BL broad ligament, bonesSpines vertebral bones, CSF cerebrospinal fluid, CT connective tissue, IQR inter quartile range, LN lymph node, lpWGS low-pass whole genome sequencing, PeriC pericardial fluid, periph peripheral, PFL pleural fluid, Q1 first quartile, Q3 third quartile, Sintestine small intestine, ST soft tissue, URN urine.