Fig. 7: Mutations detected in body liquids.

Only samples taken at autopsy are included. Only exonic and non-synonymous variants occurring in the genes of interest are considered (oncogenic genes or genes related to endocrine resistance or resistance to CDK4/6 inhibitors; see Methods). A Bar plot showing the number of mutations detected in both blood (peripheral or central) and non-blood liquids (ascites, CSF, pericardial fluid, pleural fluid and urine), as well as private mutations to either blood or non-blood liquids, for each patient. 81 samples are considered. Source data are provided as a Source Data file. B, D Heatmaps showing the mutations detected in at least one liquid for patients Pt2001 (8 samples) and Pt2003 (7 samples), respectively. The numbers in the heatmaps indicate coverage in the given sample for the given mutation. The colors indicate the VAF of the mutation in gradient blue or the absence of coverage in gray. In case multiple samples of the same body liquids were available the maximal VAF is taken. C, E Upset plots showing the mutations found in the liquids of patients Pt2001 and Pt2003, respectively. The bar plot on the bottom left shows the total number of mutations per liquid. The bar plot at the top shows the size of the intersection defined by the black dots in the matrix at the bottom. A unique dot in a column indicates the number of unique alterations for the selected liquid. ASC ascites, CSF cerebrospinal fluid, del deletion, max maximal, PeriC pericardial fluid, periph peripheral, PFL pleural fluid, URN urine, UTR untranslated region, VAF variant allele frequency.