Fig. 1: CJF-III-288 sensitizes HIV-1-infected cells to ADCC mediated by various anti-CoRBS Abs. | Nature Communications

Fig. 1: CJF-III-288 sensitizes HIV-1-infected cells to ADCC mediated by various anti-CoRBS Abs.

From: The asymmetric opening of HIV-1 Env by a potent CD4 mimetic enables anti-coreceptor binding site antibodies to mediate ADCC

Fig. 1

a Recognition and b ADCC-mediated elimination of primary CD4 + T cells infected with HIV-1CH058TF by anti-cluster A Abs (either A32 or N5i5) (n = 9) and anti-CoRBS Abs (17b) (n = 10), alone or in combination (n = 9) (at 5 µg/ml total concentration), in the presence of 50 µM of indicated CD4mc or equivalent volume of DMSO. Data are presented as mean values +/- SEM. c Recognition and d ADCC-mediated elimination of primary CD4 + T cells infected with indicated IMCs by anti-cluster A (either A32 or N5i5) and anti-CoRBS (17b), alone or in combination, in the presence of 50 µM of indicated CD4mc or equivalent volume of DMSO. c, d Shown are the median fluorescence intensities (MFI) and percentage of ADCC obtained in at least 3 biological replicates for each IMC (HIV-1CH058TF n = 10, HIV-1JRFL n = 6, HIV-1AD8 n = 3, HIV-1JRCSF n = 5). e Recognition and f ADCC-mediated elimination of primary CD4 + T cells infected with HIV-1CH058TF by indicated anti-CoRBS Abs in the presence of 50 µM of indicated CD4mc or equivalent volume of DMSO. e, f Shown are the mean MFI and percentage of ADCC obtained from 4 biological replicates for each tested anti-CoRBS Abs (17b, N12i2, X5, C2, 412D, and 48D). Statistical significance was tested using a, b Two-way ANOVA test with a Tukey post-test, c–f one-way ANOVA with a Tukey post-test or Kruskal-Wallis test with a with a Holm-Sidak post-test based on the normality test. P values are shown in the graphs. Source data are provided as a Source Data file.

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