Fig. 2: CJF-III-288 outperforms BNM-III-170 at lower concentrations. | Nature Communications

Fig. 2: CJF-III-288 outperforms BNM-III-170 at lower concentrations.

From: The asymmetric opening of HIV-1 Env by a potent CD4 mimetic enables anti-coreceptor binding site antibodies to mediate ADCC

Fig. 2

a–d Recognition of primary CD4 + T cells infected with indicated IMC by anti-CoRBS Ab (17b) alone or in combination with anti-cluster A Ab (A32), in the presence of the indicated concentration of BNM-III-170 or CJF-III-288. a, c Graphs represent the median fluorescence intensities (MFI) obtained in at least 5 biological replicates with each IMCs (HIV-1CH058TF n = 8, HIV-1JRFL n = 5, HIV-1AD8 n = 5, HIV-1JRCSF n = 5). b, d Graphs represent the area under the curve (AUC) calculated from the MFI obtained in a and c. e–h ADCC-mediated elimination of CD4 + T cells infected HIV-1CH058TF by anti-CoRBS (17b) Abs alone (n = 4) or in combination with anti-cluster A Abs (A32) (n = 5), in the presence of indicated concentration of BNM-III-170 or CJF-III-288. e, g Shown are percentage of ADCC obtained in at least 4 experiments. f, h Shown are the area under the curve (AUC) calculated from the percentage of ADCC obtained in e and g. The dashed lines represent the mean binding or ADCC obtained in absence of CD4mc. Data are presented as mean values +/- SEM. Statistical significance was tested using a, c, e, g Two-way ANOVA test with a Holm-Sidak post-test, b, d, f, h two-sided paired t test or two-sided Wilcoxon test based on the normality test. P values are shown in the graphs. Source data are provided as a Source Data file.

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