Fig. 4: The structure of the TkoTpt1/DNA/Appr > P complex-A.
From: Crystal structures and snapshots along Tpt1-catalyzed phosphate transfer from nucleic acid to NAD+
a Overall folding of the TkoTpt1/DNA/Appr>P complex-A. The bound DNA and Appr>P molecules are shown as sticks with their C-atoms colored in pink and cyan, respectively. b 2Fo-Fc electron density maps of the bound DNA and Appr>P molecules. c The detailed interactions between TkoTpt1 and the cyclic PO4 and ribose of Appr>P. d Superposition showing the conformational changes prior to and after the formation of the Appr>P product. Protein residues, DNA, and Appr>P in TkoTpt1/DNA/Appr>P complex-A are colored as in (c). For the TkoTpt1/DNA/NAD+ complex, the C-atoms of protein residues, DNA, and NAD+ are colored in gray and the sulfur atom of the bound SO42− is colored in green.
