Fig. 3: APIP promotes the caspase-11 non-canonical inflammasome activation by intracellular LPS.
a–f Effect of APIP on non-canonical inflammasome activation. Control and Apip cKO BMDMs (a–c), or WT and APIPTG/+ BMDMs (d–f) were treated with Pam3CSK4 (1 μg/ml, 4 h) and MCC950 (10 μM, 1 h), and transfected with LPS (2.5 μg/ml, 16 h). Supernatants were analyzed by LDH assay (a, d). Cell lysates were analyzed by western blotting (b, e), and blot signals quantified by densitometry (c, f). LPS (tf), LPS transfection; NT-GSDMD, N-terminal GSDMD. Data represent mean ± SD. (n = 4 independent cultures; c n = 5). g–l Effect of APIP on caspase-11 expression. Control and Apip cKO BMDMs (g–i), or WT and APIPTG/+ BMDMs (j–l) were treated with LPS (1 μg/ml, 3 h). Cell lysates were analyzed by western blotting (g, j), and signals of pro-CASP11 (p38, arrow) and APIP were quantified by densitometry (h, i, k, l). Data represent mean ± SD. (n = 4 independent cultures; h n = 3). Statistical tests: two-way ANOVA with Tukey’s multiple comparison test (a, h, k) or unpaired two-tailed Student’s t-test (c, d, f, i, l). ns, non-significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Exact p values are in the Source Data. Source data are provided as a Source Data file. arb. units, arbitrary units.
