Fig. 3: Plate- and batch-level quality control (QC) measures for screening plate data. | Nature Communications

Fig. 3: Plate- and batch-level quality control (QC) measures for screening plate data.

From: The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contexts

Fig. 3

a Schematic of data hierarchy generated from the proposed iterative high-throughput screen. b Luminescent values and coefficient of variance (CV) for fLUC (black) and nBIT (orange) signals across two batches of neurons plated at 15,000 cells per well (Batch 1) or 10,000 cells per well (Batch 2), n = 36 per plate. c Raw luminescence values (top) and CV (bottom) for negative control wells in Screen 1 ( ~ 59,840 compounds) and Screen 2 ( ~ 47,360 compounds). n = 204 and n = 148 plates with 96 or 72 samples per compound plate for Screen 1 and 2, respectively. Data are presented as mean values +/- SEM (b, c). Source data are provided as a Source Data file. Created in BioRender. Samowitz, P. (2025) https://BioRender.com/cnld7d3.

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