Fig. 4: Data from two independent HTS-like screens for SynGAP protein expression “boosters”. | Nature Communications

Fig. 4: Data from two independent HTS-like screens for SynGAP protein expression “boosters”.

From: The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contexts

Fig. 4

a One plate of screening data from Screen 1 visualized on a 2D scatter plot—nBIT (SynGAP) signal vs. fLUC (toxicity/selectivity) signal. Threshold lines set at 3X standard deviation of the mean for negative control wells. Red shaded section above corresponds up-regulator hit zone and blue shaded section below corresponds down-regulator hit zone. Gray dots appearing in hit zone did not meet established variability criteria. b All data from Screen 1 visualized on 2D scatter plot. c Zoomed in view of Fig. 3b to visualize hit compounds from Screen 1 (127 compounds = 0.21% hit rate). d One plate of screening data from Screen 2 with adjusted threshold lines. e All data from Screen 2 visualized on 2D scatter plot. f) Zoomed in view of Fig. 3d to visualize hit compounds from Screen 2 (171 compounds = 0.36% hit rate). Source data are provided as a Source Data file.

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