Fig. 2: Loss of HSP-3 or HSP-4 triggers distinct transcriptional changes in C. elegans. | Nature Communications

Fig. 2: Loss of HSP-3 or HSP-4 triggers distinct transcriptional changes in C. elegans.

From: Functionally diversified Caenorhabditis elegans BiP orthologs control body growth, reproduction, stress resistance, aging, and autophagy

Fig. 2

a Principal components analysis of L4 WT (N2) worms grown on control (pos-1), hsp-3, or hsp-4 siRNA. Volcano plots of gene transcripts on hsp-3 (b) or hsp-4 (c) siRNA relative to control. Venn diagrams depicting the number distinct and overlapping genes upregulated (d) or downregulated (e) on hsp-3 and/or hsp-4 siRNA relative to control. Linear regression analyses of genes upregulated (f) or downregulated (g) in animals grown either hsp-3 or hsp-4 siRNA. Gene ontology (GO) analyses of biological processes upregulated on hsp-3 (h) or hsp-4 (i) siRNA relative to control. Gene ontology (GO) analysis of biological processes downregulated on hsp-3 (j) or hsp-4 (k) siRNA relative to control. Plots of the relative log-fold change of gene transcripts related to indicated biological processes which are altered on hsp-3 (l) or hsp-4 (m) siRNA. For all plots a cutoff of 1.5-fold change was used. Differential gene expression was determined by performing a quasi-likelihood F-test (QLF-test) for each indicated comparison (2-sided).

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