Fig. 3: HSP-3 and HSP-4 affect reproductive behavior and body size growth in C. elegans. | Nature Communications

Fig. 3: HSP-3 and HSP-4 affect reproductive behavior and body size growth in C. elegans.

From: Functionally diversified Caenorhabditis elegans BiP orthologs control body growth, reproduction, stress resistance, aging, and autophagy

Fig. 3

Representative confocal images of day 1 adult MQD2798 (vit-2(crg9070[vit-2::gfp]); vit-1(hq503[vit-1::mCherry]) (a) and MQD2775 (vit-3(hq485[vit-3::mCherry]); vit-2(crg9070[vit-2::gfp]) (b) grown on indicated siRNA. Scale bars are 50 μm. c Timed egg laying (TEL) assay of WT worms grown on indicated siRNA and day of adulthood. Each circle represents an individual worm, and each triangle represents the average of eggs laid by all worms. d Body area quantification of WT worms grown on indicated siRNA and day of adulthood. e Body area quantification of worms overexpressing (OE) DBL-1 (BW1940, (ctIs40[dbl-1(+) + sur-5::GFP])) on indicated siRNA and day of adulthood. For d, e each circle represents an individual worm, and each triangle represents the average body size of that given biological replica. For c–e biological replicates (n = 3) are distinguished by color; triangles are average of technical replicates (circles). Source data are provided as a Source Data file. c–e Two-way ANOVA followed by Tukey’s multiple comparisons test. c page x siRNA < 0.0001; page < 0.0001; psiRNA < 0.0001. d page x siRNA < 0.0001; page < 0.0001, and psiRNA < 0.0001. e page x siRNA = 0.003; page < 0.0001; psiRNA < 0.0001. p < 0.05 is considered statistically significant. Error bars: s.d.

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