Fig. 4: HSP-3 and HSP-4 show tissue and-age specific requirements and differentially affect longevity models in C. elegans. | Nature Communications

Fig. 4: HSP-3 and HSP-4 show tissue and-age specific requirements and differentially affect longevity models in C. elegans.

From: Functionally diversified Caenorhabditis elegans BiP orthologs control body growth, reproduction, stress resistance, aging, and autophagy

Fig. 4: HSP-3 and HSP-4 show tissue and-age specific requirements and differentially affect longevity models in C. elegans.

a Survival curves of WT animals grown on indicated siRNA from hatching (n = 74 (pos-1), n = 81 (hsp-3), n = 74 (hsp-4)). b Survival curves of WT (N2), RB1104 (hsp-3(ok1083)), and VC1099 (hsp-4(gk514)) grown on pos-1 siRNA (n = 93 (N2), n = 177 (RB1104), n = 138 (VC1099)). c Survival curve of animals hypersensitive to siRNA knockdown in neurons on indicated siRNA (TU3311 (uIs60[unc-119p::YFP + unc-119p::sid-1])) (n = 69 (pos-1), n = 49 (hsp-3), n = 54 (hsp-4)). Survival curves of WT worms grown on hsp-3 or hsp-4 siRNA after larval development (d) or only during larval development (e) (For d, n = 88 (pos-1), n = 60 (hsp-3), n = 63 (hsp-4); for e, (n = 95 (pos-1), n = 116 (hsp-3), n = 83 (hsp-4)). Survival curves of worms sensitive to siRNA only in the intestines (f IG1839, (frSi17[mtl- 2p::rde-1 3’UTR]; frIs7[nlp-29p::GFP + col-12p::DsRed]; rde-1(ne300))), germline (g DCL569, (mkcSi13[sun-1p::rde-1::sun-1]; rde-1(mkc36)]), muscle (h WM118, (neIs9[myo-3::HA::rde-1 + rol-6(su1006)]; rde-1(ne300)]), or neurons (i MAH677, (sqIs71[rgef-1p::GFP + rgef-1p::sid-1]; sid-1(qt9)]) on indicated siRNA (for f, n = 61 (pos-1), n = 47 (hsp-3), n = 34 (hsp-4); for g, n = 117 (pos-1), n = 108 (hsp-3), n = 103 (hsp-4); for h, n = 63 (pos-1), n = 80 (hsp-3), n = 71 (hsp-4); for i, n = 19 (pos-1), n = 59 (hsp-3), n = 38 (hsp-4)). j Survival curve of DA1116 (eat-2(ad1116)) on indicated siRNA (n = 101 (pos-1), n = 125 (hsp-3), n = 89 (hsp-4)). k Survival curve of KAE9 (seaSi40[eft-3p::fmo-2 + H2B::GFP] + [Cbr-unc-119(+)]; unc-119(ed3)]) on indicated siRNA (n = 100 (pos-1), n = 105 (hsp-3), n = 49 (hsp-4)). l Survival curve of CB1370 (daf-2(e1370)) grown on indicated siRNA (n = 142 (pos-1), n = 106 (hsp-3), n = 73 (hsp-4)). Log-rank Mantel-Cox test. p < 0.05 is considered statistically significant.

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